Antigenic analysis of coxsackievirus B3 with monoclonal antibodies.

Serial immunologic identification of lymphocyte subsets in murine coxsackievirus B3 myocarditis: different kinetics and significance of lymphocyte subsets in the heart and in peripheral blood.

To elucidate possible immune mechanisms in the pathogenesis of myocarditis, we examined, by immunofluorescence techniques, the serial changes in lymphocyte subsets in the heart, spleen, and peripheral blood of C3H/He mice inoculated coxsackievirus B3 (experiment I). B cells were identified by staining with fluorescein isothiocyanate-labeled rabbit antimouse immunoglobulin. T cell subsets were d...

Monoclonal antibody therapy for prevention of acute coxsackievirus B3 myocarditis in mice.

The efficacy of monoclonal antibodies against T cell subsets in the therapy of experimental myocarditis caused by coxsackievirus B3 (CB3) was investigated. Two-week-old male C3H/He mice were inoculated with CB3 virus. Treatment was begun in the viremic stage (starting on the day of inoculation) in experiment 1 and in the later aviremic stage (starting on day 10) in experiment 2. Rat anti-mouse ...

Roles of programmed death-1 (PD-1)/PD-1 ligands pathway in the development of murine acute myocarditis caused by coxsackievirus B3.

OBJECTIVE This study was designed to investigate the roles of programmed death-1 (PD-1) and PD-1 ligands (PD-L) in the development of murine acute myocarditis caused by Coxsackievirus B3. PD-1/PD-L belong to the CD28/B7 superfamily, and the PD-1/PD-L pathway is known to transduce a negative immunoregulatory signal that antagonizes the T-cell receptor-CD28 signal and inhibits T-cell activation. ...

Effect of Host Src-kinase Inhibition on Replication of Coxsackievirus B3 in vitro

Application of Specific Monoclonal Antibodies for Characterization of Leishmania spp. Promastigotes Using Indirect Immunofluorescent and Immunoperoxidase Tests

Background: Different methods have been used for characterization of Leishmania promastigotes. Monoclonal antibodies are useful in characterization of   Leishmania spp . both amastogotes and promastigotes. Objective: Comparing the characterization of   Leishmania spp. promastigotes with immunoperoxidase test (Avidin-Biotin) techniques and an indirect immunofluorescent assay (IFA).   Methods: Ap...